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Enzyme-immobilized carrier, and immobilized lipase.
Enzyme-immobilized carrier, and immobilized lipase.
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机译:固定化酶的载体和固定化脂肪酶。
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摘要
A carrier for immobilizing lipase is prepared by bonding a silane coupling agent having a carboxylic-ester group to an inorganic carrier. Preferred silane coupling agents are gamma -methacryl oxypropyltrimethoxy silane, gamma -acetoxypropyltrimethoxy silane or gamma -acryloxypropyltrimethoxy silane. The inorganic carrier can be kaolinite, porous glass, bentonite, silica gel, alumina, silica, silica-alumina hydroxy apatite or calcium phosphate gel. Lipase is immobilized by mixing lipase with the inorganic carrier containing the bound silane coupling agent, filtering the resultant mixture and drying the filtered mixture. Functional groups of the lipase are bonded to the carboxylic-ester group of the silane coupling agent.
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