首页>
外国专利>
Purification of DNA samples detection of polymorphisms and mutations, have been amplified, and for the identification of alleles, the use of immobilized mismatch binding protein
Purification of DNA samples detection of polymorphisms and mutations, have been amplified, and for the identification of alleles, the use of immobilized mismatch binding protein
展开▼
机译:DNA样品的纯化检测多态性和突变,已被扩增,并用于鉴定等位基因,使用固定的错配结合蛋白
展开▼
页面导航
摘要
著录项
相似文献
摘要
A method for detecting mutations, such as a single base change or an addition or deletion of about one to four base pairs, is based on the use of an immobilized DNA mismatch-binding protein, such as MutS, which binds to a nucleic acid hybrid having a single base mismatch or unpaired base or bases, thereby allowing the detection of mutations involving as little as one base change in a nucleotide sequence. Such a method is useful for diagnosing a variety of important disease states or susceptibilities, detecting the presence of a mutated oncogene and for isolating or removing by affinity chromatography duplex DNA molecules containing mismatches such as error-containing molecules in PCR-amplified DNA samples. Also provided are kits useful for practicing the methods of the present invention.
展开▼