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Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification
Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification
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机译:固定错配结合蛋白在检测突变和多态性以及等位基因鉴定中的用途
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摘要
A method for detecting mutations, such as a single base change or an addition or deletion of about one to four base pairs, is based on the use of an immobilized DNA mismatch-binding protein, such as MutS, which binds to a nucleic acid hybrid having a single base mismatch or unpaired base or bases, thereby allowing the detection of mutations involving as little as one base change in a nucleotide sequence. Such a method is useful for diagnosing a variety of important disease states or susceptibilities, including the presence of a mutated oncogene and the presence of DNA containing triplet repeat sequences which characterize several genetic diseases including fragile X syndrome. The present method is used to isolate or remove by affinity chromatography duplex DNA molecules containing mismatches such as error-containing molecules in PCR- amplified DNA samples. Also provided are compositions and kits useful for practicing the methods of the present invention.
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