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It depends on the ionicity surface active agent, suppression manner of the inhibition for the reaction which is mediated by the enzyme
It depends on the ionicity surface active agent, suppression manner of the inhibition for the reaction which is mediated by the enzyme
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机译:取决于离子表面活性剂,酶介导的反应抑制的抑制方式
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摘要
Methods and kits for the use of a non-ionic detergent to suppress enzyme inhibition in a reaction solution due to the presence of inhibiting ionic detergent. Prior to reaction, the reaction mixure is given an effective amount of a non-ionic detergent, and agitated. The enzyme is then added, and the enzymatic reaction is the allowed to proceed. Also disclosed are preferred embodiments of the present invention, and kits for nucleic acid amplification of a biological sample in the presence of an ionic detergent. IMAGE
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