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Method for suppressing inhibition of enzyme-mediated reactions by ionic detergents using high concentration of non-ionic detergent
Method for suppressing inhibition of enzyme-mediated reactions by ionic detergents using high concentration of non-ionic detergent
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机译:使用高浓度的非离子型洗涤剂抑制离子型洗涤剂对酶介导反应的抑制的方法
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摘要
Methods and kits for the use of a non-ionic detergent to suppress enzyme inhibition in a reaction solution due to the presence of inhibiting ionic detergent. Prior to reaction, the reaction mixture is given an effective amount of a non-ionic detergent, and agitated. The enzyme is then added, and the enzymatic reaction is the allowed to proceed. Also disclosed are preferred embodiments of the present invention, and kits for nucleic acid amplification of a biological sample in the presence of an ionic detergent.
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