CDNA OF NEW POLYPEPTIDE GENE, VECTOR CONTAINING THE SAME CDNA, HOST CELL TRANSFORMED WITH THE SAME VECTOR, POLYPEPTIDE PRODUCED THEREBY, ITS PRODUCTION, DNA CAPABLE OF CODING THE SAME POLYPEPTIDE AND ANTIBODY AGAINST THE SAME POLYPEPTIDE

摘要

PROBLEM TO BE SOLVED: To obtain a new cDNA, capable of coding a polypeptide produced by a mantle tissue of Pinctada fucata martensii Dunker, having a specific base sequence and capable of providing a polypeptide useful for development of a new raw material for cosmetics or a new method, etc., for selecting a piece in pearl culture. ;SOLUTION: This new cDNA is capable of coding a polypeptide produced by a mantle epithelial tissue of Pinctada fucata martensii Dunker and used for genetic engineering production, etc., of a new polypeptide useful for developing a new raw material for cosmetics or development, etc., of a new method for selecting a piece in pearl culture. The new cDNA is obtained by isolating an mRNA from a mantle epithelial tissue of Pinctada fucata martensii Dunker, synthesizing a cDNA with a reverse transcription enzyme using the mRNA as a template, preparing a cDNA library therewith according to a conventional method, then screening the resultant cDNA library with a synthetic nucleotide capable of coding a part of an insoluble protein of a pearl layer of the Pinctada fucata martensii Dunker as a probe and recovering the cDNA from a positive clone.;COPYRIGHT: (C)1997,JPO