CDNA of the novel polypeptide gene, a vector containing the cDNA, the vector host cell transformed with, whereby the polypeptide is produced, a manufacturing method thereof, DNA encoding the polypeptide, and the polypeptide of the antibodies

摘要

PROBLEM TO BE SOLVED: To obtain a new cDNA, capable of coding a polypeptide produced by a mantle tissue of Pinctada fucata martensii Dunker, having a specific base sequence and capable of providing a polypeptide useful for development of a new raw material for cosmetics or a new method, etc., for selecting a piece in pearl culture. ;SOLUTION: This new cDNA is capable of coding a polypeptide produced by a mantle epithelial tissue of Pinctada fucata martensii Dunker and used for genetic engineering production, etc., of a new polypeptide useful for developing a new raw material for cosmetics or development, etc., of a new method for selecting a piece in pearl culture. The new cDNA is obtained by isolating an mRNA from a mantle epithelial tissue of Pinctada fucata martensii Dunker, synthesizing a cDNA with a reverse transcription enzyme using the mRNA as a template, preparing a cDNA library therewith according to a conventional method, then screening the resultant cDNA library with a synthetic nucleotide capable of coding a part of an insoluble protein of a pearl layer of the Pinctada fucata martensii Dunker as a probe and recovering the cDNA from a positive clone.;COPYRIGHT: (C)1997,JPO