RIBOZYME HAVING TRNA ANTICODON STEM LOOP

摘要

PROBLEM TO BE SOLVED: To obtain a new subject ribozyme having a tRNA anticodon stem loop in a molecule, stable in a living body, capable of specifically breaking a natural RNA or the like giving a harmful influence on a living body and useful for prevention and treatment of AIDS, tumor, etc. ;SOLUTION: This new ribozyme is composed of a polyribonucleotide containing a nucleotide sequence of the formula (U is a uracil nucleotide; C is a cytosine nucleotide; A is an adenine nucleotide; G is a guanine nucleotide; S is U, C, G; Q₁-Q_n are each U, A, C, G; R₁-R_n are each a complementary nucleotide to Q₁-Q_n;W₁-W_k are each U, A, C, G; X₁-X_m are each U, A, C, G; D₁-D₃ are each U, A, C, G; (e) is 5-10; (k), (m) and (n) are each 1-12. The hair pin loop comprising D₁-D_e, Q₁-Q_n and R₁-R_n is composed of a tRNA anticodon stem loop of unmodified nucleotide sequence) and possible to specifically cleave a natural polyribonucleotide or RNA giving a harmful influence against a living body.;COPYRIGHT: (C)1997,JPO