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Process for selective removal of salivary alpha-amylase and assay for pancreatic alpha-amylase
Process for selective removal of salivary alpha-amylase and assay for pancreatic alpha-amylase
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机译:选择性去除唾液α-淀粉酶的方法和测定胰α-淀粉酶的方法
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摘要
Inter alia, a process for the selective removal of salivary alpha -amylase from a sample comprising salivary alpha -amylase and pancreatic alpha -amylase characterised in that there is used a monoclonal antibody against salivary alpha -amylase, which is immobilised or is coupled to a physically seperable or seperate support and which exhibits a binding affinity towards salivary alpha -amylase of at elast 1 x 107 l/m and a cross-reactivity with pancreatic alpha -amylase of less than 1% is disclosed. The remaining pancreatic alpha -amylase may be assayed.
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机译:尤其是,从包含唾液α-淀粉酶和胰腺α-淀粉酶的样品中选择性去除唾液α-淀粉酶的方法,其特征在于使用固定化或偶联至唾液α-淀粉酶的单克隆抗体来唾液α-淀粉酶。公开了在物理上可分离或可分离的载体,其对唾液α-淀粉酶的结合亲和力至少为1×10 7 l / m,并且与胰α-淀粉酶的交叉反应性小于1%。可以检测剩余的胰腺α-淀粉酶。
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