Changes Occurring on the Activity of Salivary Alpha-Amylase Proteoforms in Two Naturalistic Situations Using a Spectrophotometric Assay

摘要

Salivary alpha-amylase (sAA) is considered a biomarker of acute stress since this enzyme is released in saliva after autonomic nervous system activation, in response to psychological or physical stress situations. This enzyme has different isoforms that could be differentially expressed depending on the stressful situation. The aims of the present research were (1) to develop and validate an easy and fast method to estimate the activity of the major sAA proteoforms (both non-glycosylated and glycosylated proteoforms) in saliva samples, and (2) to evaluate the possible changes occurring in the activity of both proteoforms when measured by this method in two different stress models (physical effort and psychological challenge). This new method was precise and, when applied to the different stress models, allowed to detect changes of different magnitudes in both proteoforms. Therefore, this research opens a new field for the evaluation of isoforms of sAA as potential biomarkers of stress.