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METHOD FOR PRODUCING STREPTOMYCES MUTANTS, FOR ISOLATING THE MUTANT AND THE CORRESPONDING NATURAL GENES, AND IDENTIFYING MOBIL GENETIC ELEMENTS BY USING TEMPERATURE SENSITIVE HIBRID PLASMIDS
METHOD FOR PRODUCING STREPTOMYCES MUTANTS, FOR ISOLATING THE MUTANT AND THE CORRESPONDING NATURAL GENES, AND IDENTIFYING MOBIL GENETIC ELEMENTS BY USING TEMPERATURE SENSITIVE HIBRID PLASMIDS
The streptomycetes plasmid pSG5 is temperature-sensitive. It is thus possible with the aid of the pSG5 replicon to prepare temperature-sensitive hybrid plasmids. The latter are suitable for screening for DNA segments which are homologous with DNA segments of the hybrid plasmid.
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