ribosome inactivating proteins, called alpha-nigritina, beta-and gamma-nigritina nigritina, Sambucus nigra plant l., process for their preparation and their use.

摘要

"Ribosome inactivating proteins, called alpha-NIGRITINA, BETA-GAMMA-NIGRITINA NIGRITINA Y, PLANT Sambucus nigra L., process for their preparation and their use." ALPHA-NIGRITINA, BETA-GAMMA-NIGRITINA NIGRITINA And, RELATIVE MOLECULAR MASSES PRESENTS 29000, 40000 and 27500 daltons respectively. ALFA-NIGRITINA the following sequence of amino acids at its amino-terminal: ALA-SER-VAL-LEU-ASP-SER-ILE-GLN-PHE-VAL-SER-LEU-GLU-LYS-XXX-ALA-Val- TYR-GLU-GLN-ASN. GAMMA-NIGRITINA has three tryptic peptides WITH following amino acid sequence: A) VAL-THR-ASN-SER-PHE-TYR-LEU B) TYR-PRO-SER-XXX-ILE-ILE-ARG C) TRP-XXX- ASN-THR-SER-TYR-HIS-ASN-LEU-GLU-ILE-PHE-ILE-XXX-LEU. THE GENERAL PROCEDURE FOR OBTAINING RIPs Sambucus nigra of the present invention comprises isolating THE SAME SHEETS Sambucus nigra including first extraction operation with an aqueous solution based on sodium chloride and monosodium phosphate for obtaining an extract be able to inhibit protein synthesis, followed by purification of the extract by chromatography techniques MOLECULAR EXCLUSION ion exchange. ALPHA-NIGRITINA, BETA-NIGRITINA, and gamma- NIGRITINA USED AS inactivator ribonucleic acid and inhibitor of protein biosynthesis.