ribosome inactivating proteins, called nigrina nigrina 11 and 12, Sambucus nigra plant l., process for their preparation and obtainment.

摘要

Ribosome inactivating proteins, called nigrina 11 nigrina 12 Sambucus nigra PLANT 11 METHOD FOR OBTAINING AND PREPARATION. Nigrin 11 nigrina 12 type 2 (comprising two POLIPETIDICAS CADENAS) RELATIVE MOLECULAR MASSES PRESENTS 59500 Daltons, IN THE ABSENCE OF REDUCING. PRESENTED BY TWO CHAINS A chain disulfide bridges UNITED IN THE PRESENCE OF REDUCING MR HAVE A 33500 and 39000 daltons FOR STRINGS B, 26000 AND CHAIN ​​A. PRESENTED FOR THE FOLLOWING STREAMS amino acids at its N-terminal: nigrina 11 : CHAIN ​​A: ILE-TYR-ASP-SER-VAL-PRC-SER-PHE-ASN-LEU-ASP-GLY-ALA-LYS-SER-TYR-ARG-ALATHR-ASP B CHAIN: ASP-GLY-Glu- THR-XAA-THR-LEU-ARG-THR-SER-PHE-THR-ARG-ASN-ILE-VAL-GLY-XAA-ASP-GLY nigrina 12: CHAIN ​​A: ILE-ASP-TYR-PRO-SER-VAL SER-PHE-ASN-LEU-ASP-GLY-ALA-LYS-SER-ALA-THR-TYR-ARG-ASP-PHE-LEU-SERASN CHAIN ​​B: ASP-GLY-GLU-THR-THR-LEU-XAA -ARG-THR-SER-PHE-THR-ASN-ARG-ILE-VAL-GLY-ASP-GLY-XAA PROCEDURE GENERALPARA oBTAINING RIPs Sambucus nigra L. of the present invention comprises isolating SHOOTS THE SAME DE Sambucus nigra L., including first extraction operation with an aqueous solution BASED AND SODIUM CHLORIDE monosodium phosphate to obtain an extract which is capable of inhibiting Protein synthesis, purification thereof FOLLOWED BY TECHNIQUES affinity chromatography and ion exchange MOLECULAR EXCLUSION. Nigrin L1 and L2 nigrina USED AS inactivator ribonucleic acid and inhibitor of protein biosynthesis.