ribosome inactivating proteins, called alpha-ebulitina, beta-and gamma-ebulitina ebilitina, the Sambucus plant ebulus, l., process for their preparation and their use.

摘要

Ribosome inactivating proteins, called alpha-EBULITINA, BETA-GAMMA-EBULITINA EBULITINA Y, PLANT Sambucus Ebulus L., process for their preparation and their use. ALPHA-EBULITINA, BETA-GAMMA-EBULITINA EBULITINA and has the following amino acid composition: ALFA-EBULITINA: CYS-0.0; ASP-34.7; THR-26.5; BE-20.7; GLU-36.4; PRO-14.4; GLY-23.6; ALA-21.1; VAL-22.7; MET-4,5; ILE-14.4; LEU-28.1; TYR-6,6; PHE-9,9; LYS-8,6; HIS-3,7; ARG-16,1. BETA-EBULITINA: CYS-6,1; ASP-36.4; THR-31.2; BE-14.6; GLU-20.7; PRO-15.2; GLY-16.6; ALA-21.3; VAL-12.3; MET-2,1; ILE-15.4; LEU-19.2; TYR-8,8; PHE-5,7; LYS-8,5; HIS-8,3; ARG-18,6. GAMMA-EBULITINA: CYS-6,0; ASP-31.1; THR-33.1; BE-15.5; GLU-21.5; PRO-14.5; GLY-18.3; ALA-21.5; VAL-9,2; MET-4,0; ILE-18.0; LEU-17.8; TYR-7,2; PHE-7,7; LYS-6,2; HIS-9,2; ARG-20.0. THE GENERAL PROCEDURE FOR OBTAINING RIPs Sambucus Ebulus of the present invention comprises isolating THE SAME SHEETS Sambucus Ebulus INCLUDING UNAPRIMERA OPERATION OF REMOVING AN AQUEOUS SOLUTION BASED sodium chloride and monosodium phosphate for obtaining an extract be able to inhibit protein synthesis, followed by purification of the extract by chromatography techniques IONICO EXCHANGE OF EXCLUSION MOLECULAR AND AFFINITY. ALPHA-EBULITINA, BETA-GAMMA-EBULITINA EBULITINA AND USED AS inactivator ribonucleic acid and inhibitor of protein biosynthesis.