A diffusion chamber system including in combination an Ussing diffusion chamber (10) and fluid circulating device (18) for transport studies using membranous barriers such as excised animal tissue cultered cell monolayers. The chamber is provided with recesses (32, 34) in two mating chamber halves (20, 22) to receive a barrier support on an insert (36). Potential electrodes (114, 116) extend obliquely through the walls of the chamber halves (20, 22) and into both ends of the insert (36) so that the tips (128, 130) of the electrodes (114, 116) are in close proximity to, and centered on, the barrier.
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