Human rhinovirus receptor protein (ICAM-1) that inhibits rhinovirus attachment and infectivity

摘要

A water soluble human rhinovirus (HRV) major receptor preparation comprising detergent-complexed glycoprotein isolated from animal cells, preferably mammalian cells, that express the HRV major receptor and which exhibits the ability to bind to HRV capsids to substantially reduce infectivity of the virus. The purified, water soluble receptor is obtained by extracting cells expressing the receptor with detergent and isolating the solubilized detergent-glycoprotein complexes by binding to monoclonal antibody selective for the HRV receptor protein. Human rhinovirus receptor protein has subsequently been discovered by Greve et al. to be ICAM-1.