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Method for making recombinant yeast artificial chromosomes by minimizing diploid doubling during mating
Method for making recombinant yeast artificial chromosomes by minimizing diploid doubling during mating
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机译:通过使交配期间的二倍体倍增最小化来制备重组酵母人工染色体的方法
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摘要
The present invention provides methods for construction of recombinant Yeast Artificial Chromosomes ("YAC") by homologous recombination between YACs during meiosis. In particular, conditions are provided for the step of mating haploid cells and for the step of spore analysis that increase the frequency of spores containing the desired recombinant YAC. The methods find particular use in constructing recombinant YACs between YACs that are incompatible when co-propagated in a diploid and/or that share homology regions of less than about 50 kilobases. Linking YACs, methods of their construction, and methods of their use are provided that allow facile construction of a YAC containing two or more discontinuous regions of DNA.
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