Method of making a recombinant yeast artificial chromosome

摘要

(57) [Abstract] The present invention provides a method for making ("YAC") recombinant yeast artificial chromosomes by homologous recombination between the YAC during meiosis. In particular, conditions for spore analysis step and the step of mating the haploid cells to increase the frequency of spores containing the recombinant YAC that is required is provided. Are incompatible if it is simultaneously grown in a diploid, the method is used and in particular to generate recombinant YAC of YAC in mutually separate the homologous region of about 50 kilobases or less than /. Consolidated YAC that allows easy production of YAC, including the discontinuous two or more regions of DNA, its how to make and use that time is today.