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Methods for producing recombinant mammalian cells harboring a yeast artificial chromosome
Methods for producing recombinant mammalian cells harboring a yeast artificial chromosome
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机译:产生具有酵母人工染色体的重组哺乳动物细胞的方法
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摘要
The present invention provides methods and compositions for transferring large transgene polynucleotides and unlinked selectable marker polynucleotides into eukaryotic cells by a novel method designated co-lipofection. The methods and compositions of the invention are used to produce novel transgenic non-human animals harboring large transgenes, such as a transgene comprising a human APP gene or human immunoglobulin gene.
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