Manufacturing method null of the useful material which uses the microbe and the said microbe which possess the high manifestation vector and the said high manifestation vector

摘要

PURPOSE: To provide a new high-expression vector useful for the production of an exogenote product. ;CONSTITUTION: A high-expression vector pHT having HWP promoter and a multicloning site of formula, e.g. pHT210. A plasmid having a DNA fragment produced by linking an α-amylase (BLA) gene of Bacillus licheniformis to the downstream of an MWP promoter region of Bacillus brevis 47 (FERM P-7224) is treated with a restriction enzyme, the HWP promoter region fragments extracted from the plasmid are linked together, a Bacillus brevis is transformed to obtain a plasmid containing HWP promoter in place of MWP promoter and the obtained plasmid is subjected to restriction enzyme treatment, ligase treatment and linker treatment to obtain a high-expression vector free from BLA gene.;COPYRIGHT: (C)1994,JPO&Japio