Manufacturing method null of the useful material which uses the microbe and the said microbe which possess the revelation control vector and the said vector

摘要

PURPOSE:To obtain an expression controlled vector pNU550 capable of producing a useful exogenote product when bonded with an exogenote by linking a lac repressor originated from E.coli to the downstream of MWP promoter originated from Bacillus brevis. CONSTITUTION:A DNA containing an MWP promoter region of Bacillus brevis 47 (FERM P-7224) is cut with a restriction enzyme. A lac repressor gene originated from E.coli is linked to the downstream of the above fragment to obtain an expression controlled vector pNU550 expressed by the restriction enzyme map of fig.1. The expression controlled vector pNU550 is treated with a restriction enzyme and bonded to a gene of an exogenote product such as human salivary gland amylase gene or alpha-amylase gene of Bacillus licheniformis to effect the transformation of a host and obtain an expression controlled vector pNU550-X for the production of a useful exogenote product (e.g. human salivary gland amylase).