Radiolabeled o-methyl phosphotriester and o-methyl phosphorothioate internucleoside linkages and oligonucleotides containing the same

摘要

The invention provides oligonucleotides containing methyl phosphotriester linkages and processes for making and methods for using such oligonucleotides. The preparation of radiolabeled oligonucleotides is also disclosed. The radiolabel is a 14C-methyl group attached to an oxygen atom of an internucleoside phosphate group.