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Two-stage continuous cell culture method for production of intracellular secondary metabolites
Two-stage continuous cell culture method for production of intracellular secondary metabolites
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机译:两阶段连续细胞培养法生产细胞内次生代谢产物
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摘要
The present invention relates to a process capable of greatly improving the productivity in the production of intracellular secondary metabolites. More specifically, the present invention proposes a method for dramatically improving productivity through continuous culture using a fixed-bed cell culture incubator instead of simple batch culture, furtenhancing productivity by adding a suspension culture system to the two-stage culture The process technology. (I) a step of using a continuous fixed bed cell culture apparatus for production of intracellular secondary metabolites; (ii) Including a fixed cell incubator as a single step and a suspension cell incubator as a two-step incubator. The continuous production process of the intracellular secondary metabolites of the present invention enables a much higproductivity than the continuous process through the conventional suspension incubator and furtimproves the productivity by adopting the two-stage process.
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