METHOD OF IDENTIFYING A CELL WITH AN INTRACELLULAR CONCENTRATION OF A SPECIFIC METABOLITE, WHICH INTRACELLULAR CONCENTRATION IS INCREASED IN COMPARISON WITH THE CELL'S WILDTYPE, WHERE THE MODIFICATION OF THE CELL IS ACHIEVED BY RECOMBINEERING, AND METHOD OF MAKING A PRODUCTION CELL WITH OPTIMIZED PRODUCTION OF A SPECIFIC METABOLITE, WHICH PRODUCTION CELL IS GENETICALLY MODIFIED IN COMPARISON WITH ITS WILDTYPE, METHOD OF PRODUCING THIS METABOLITE, AND NUCLEIC ACIDS WHICH ARE SUITABLE THEREFOR

摘要

A method for identifying a cell having an intracellular concentration of a particular metabolite that is increased compared to the wild type of the cell, wherein the modification of the cell is achieved by recombineering, and to a method for producing a production cell that is genetically modified compared to the wild type of the cell and has optimized production of a particular metabolite, to a method for producing this metabolite, and to nucleic acids suited therefor. A gene coding for a recombinase, which is homologous to a known recombinase gene, is transformed in a cell using a vector, and a DNA containing at least one modified gene G1 to Gn, or at least one mutation M1 to Mn, is inserted into the cell, and the cell that has highest metabolite production is identified by way of metabolite sensors. A mutation, which is considered to the cause for the increased production, is isolated from this cell, and the gene or the mutation is removed and inserted into a production strain, which thereby exhibits increased production of the metabolite.