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RECOMBINANT PLASMID DNA PRO PF5 DETERMINING SYNTHESIS OF CAPSULE ANTIGEN FI OF PLAGUE PATHOGEN AND PROTECTIVE ANTIGEN OF ANTHRAX PATHOGEN AND A METHOD OF CONSTRUCTION OF RECOMBINANT PLASMID DNA PRO PF5
RECOMBINANT PLASMID DNA PRO PF5 DETERMINING SYNTHESIS OF CAPSULE ANTIGEN FI OF PLAGUE PATHOGEN AND PROTECTIVE ANTIGEN OF ANTHRAX PATHOGEN AND A METHOD OF CONSTRUCTION OF RECOMBINANT PLASMID DNA PRO PF5
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机译：重组质粒DNA PRO PF5测定鼠疫病原性胶囊抗原FI和炭疽病预防性抗原的合成及重组质粒DNA PF5的构建方法
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FIELD: molecular biology, biotechnology, microbiology. SUBSTANCE: plasmid pro PF5 is obtained by association of plasmid pro P5-fragment encoding protective antigen of anthrax pathogen and plasmid pKMI-fragment containing fra-operon with gene encoding capsule antigen FI of plague pathogen. Recombinant plasmid DNA pro PF5 is inherited constantly in E. coli JM83. After 80 generations on L-broth without selective pressure stability is 100%. EFFECT: improved method of recombinant plasmid construction. 2 cl, 2 dwg
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机译：领域：分子生物学，生物技术，微生物学。物质：通过编码炭疽病病原体保护性抗原的质粒pro P5片段和含有fra操纵子的质粒pKMI片段与编码鼠疫病原体荚膜抗原FI的基因相关联，获得了质粒pro PF5。重组质粒DNA pro PF5在大肠杆菌JM83中不断遗传。在不选择压力的情况下，对L汤进行80代试验后，稳定性为100％。效果：改进的重组质粒构建方法。 2 cl，2 dwg
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