RECOMBINANT PLASMID DNA PESG, METHOD OF PREPARING RECOMBINANT PLASMID DNA PESG AND STRAIN OF BACTERIUM ESCHERICHIA COLI CONTAINING RECOMBINANT PLASMID DNA PESG USED FOR PREPARING THE HYBRID PROTEIN CONSISTING OF 485 AMINO ACIDS SHOWING ANTIGENIC PROPERTIES OF HUMAN VIRUS T-CELLULAR LEUKOSIS OF THE FIRST TYPE

摘要

FIELD: biotechnology, genetic engineering. SUBSTANCE: invention relates to methods for detection of human virus T-cellular leukosis of the first type (HTLV-1). Invention involves preparing recombinant plasmid DNA providing high level of hybrid env-protein HTLV-1 production showing antigenic properties and preparing the strain-producer of the protein indicated. Plasmid pESG is obtained by fusion of plasmid pVR-291 fragment encoding E. coli β-galacosidase, two polylinkers, start replication region and gene determining resistance to ampicillin with plasmid pBEI fragment containing the sequence for env-protein HTLV-1. E. coli cells were transformed with the obtained plasmid and the strain GKV/pESG producing hybrid protein and containing specific virus-specific sequences of the gene env HTLV-1 is selected. Yield of protein showing antigenic properties of HTLV-1 at culturing the strain GKV/pESG is 200-350 mg/1 l cell suspension. EFFECT: improved method of plasmid preparing, increased yield of protein. 3 cl, 3 dwg