RECOMBINANT PLASMID DNA PYH1 - A SOURCE OF F2 DNA-PROBE FOR IDENTIFICATION OF PLAGUE MICROBE STRAIN WITH CAPSULE ANTIGEN GENES, STRAIN OF BACTERIUM ESCHERICHIA COLI - A DONOR OR RECOMBINANT PLASMID DNA PYH1 CONTAINING F2 DNA-PROBE FOR IDENTIFICATION OF PLAGUE MICROBE STRAIN WITH CAPSULE ANTIGEN GENES

摘要

FIELD: microbiology. SUBSTANCE: recombinant plasmid pYH1 with DNA-fragment of plasmid pFra is constructed, the size is about 800 nucleotide pairs and replicon of vector plasmid pACYC 184. Prepared DNA is used for transformation of E. coli cells K 802, and strain containing recombinant plasmid pYH1 is constructed. In the process of replication of this plasmid DNA synthesis of F2 probe is carried out. DNA F2 probe which is a part of pFra plasmid ensures to identify plague microbe strains containing genes of capsule antigene F₂. Probe F2 shows species specificity, it is not hybridized with DNA of yiersinia of other species and with other representatives of family Enterobacteriacae (E. coli, shigellae, salmonellae). Constructed strain of E. coli has recombinant plasmid DNA carrying fragment of plasmid pFra of plague microbe. EFFECT: construction of bacterial strain indicated above. 2 cl, 1 tbl