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Detection of maize fungal pathogens using the polymerase chain reaction
Detection of maize fungal pathogens using the polymerase chain reaction
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机译:利用聚合酶链反应检测玉米真菌病原体
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摘要
Internal Transcribed Spacer (ITS) DNA sequences from the ribosomal RNA gene region are described for different species and strains of Helminthosporium carbonum, Helminthosporium turcicum, Helminthosporium maydis, Cercospora zeae-maydis, Kabatiella zeae and Puccinia sorghi. Specific primers from within these sequences are identified as being useful for the identification of the fungal isolates using PCR-based techniques.
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