Detection of maize fungal pathogens using the polymerase chain reaction

摘要

Internal Transcribed Spacer (ITS) DNA sequences from the ribosomal RNA gene region are described for different species and strains of Helminthosporium carbonum, Helminthosporium turcicum, Helminthosporium maydis, Cercospora zeae-maydis, Kabatiella zeae and Puccinia sorghi. Specific primers from within these sequences are identified as being useful for the identification of the fungal isolates using PCR-based techniques.