PROBLEM TO BE SOLVED: To enable the easy and reliable detection of a qualitative and quantitative anomaly in an organism component by measuring the bioactivity of a bioactive substance in a sample captured on a solid phase by an antibody, and measuring the amount of substance by an immunological means. ;SOLUTION: A bioactive substance in a sample is brought into reaction with an antibody immobilized to a solid phase to combine a target substance with the solid phase. The bioactivity of the target substance combined with the solid phase is measured, and then the amount of substance is immunologically measured through the use of a secondary antibody labeled with the amount of antigen of the substance. Here, in the case that a substance to be measured in the sample has less bioactivity in comparison with the immunologically measured amount of substance, it indicates a qualitative anomaly in an organism component. In the case that the immunologically measured amount of the substance to be measured is extremely less or more in comparison with a normal sample, it indicates a qualitative anomaly in the organism component. At this time, an antibody to be combined with the solid phase may be a polyclonal antibody or a monoclonal antibody, and it is possible to measure a quantitative and qualitative anomaly in the organism component.;COPYRIGHT: (C)1999,JPO
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