Production manner null of the phosphoric acid content acidic sugar chain which utilizes the mannose 1 phosphotransferase gene of the yeast

摘要

PROBLEM TO BE SOLVED: To produce the subject saccharide chain to be a labeling marker in a cell by culturing a yeast cell transformed with a plasmid containing a mannose-1-phosphate transferase gene, reacting the resultant product with a neutral core saccharide chain and then treating the prepared saccharide chain with an acid. ;SOLUTION: A yeast cell transformed with a plasmid DNA containing all or a part of a mannose-1-phosphate transferase gene of a yeast (e.g. Saccharomyces cerevisiae), having an amino acid sequence represented by the formula or an amino acid sequence in which one or a plurality of amino acid residues are inserted, deleted or replaced in the sequence and capable of coding for an amino acid sequence capable of providing the enzymic activities of the mannose-1-phosphate transferase is cultured in a culture medium to provide a mannose-1-phosphate transferase gene from the resultant cultured product, which is then reacted with a neutral core saccharide chain in vivo or in vitro to afford a mannose-1-phosphate-containing acidic saccharide chain. The resultant acidic saccharide chain is treated with an acid to excise the mannose part. Thereby, the subject saccharide chain useful as a labeling marker, etc., for glycoprotein transport to a lyzozyme is obtained.;COPYRIGHT: (C)1998,JPO