MANNOSE 1-PHOSPHATE TRANSFERASE GENE OF YEAST AND ACIDIC SACCHARIDE CHAIN CONTAINING MANNOSE 1-PHOSPHATE AND PRODUCTION OF ACIDIC SACCHARIDE CHAIN CONTAINING PHOSPHORIC ACID UTILIZING THE SAME

摘要

PROBLEM TO BE SOLVED: To obtain the subject new gene, capable of coding a mannose 1-phosphate transferase of a yeast having a specific amino acid sequence and useful for production, etc., of an enzyme capable of providing a saccharide chain as a labeling marker for transporting a glycoprotein to a lysosome ion a mammalian cell. ;SOLUTION: This mannose 1-phosphate transferase gene is a new gene capable of coding the mannose 1-phosphate transferase of a yeast (Saccharomyces cerevisiae) having an amino acid sequence represented by the formula and capable of mass-producing an acidic saccharide chain having a mannose 1-phosphate added to the same neutral core saccharide chain as a high-mannose type neutral saccharide chain produced by a mammalian cell in a good purity and further useful for producing the mannose 1-phosphate transferase providing the acidic saccharide chain capable of functioning as a labeling marker for transporting a glycoprotein to a lysosome in a mammalian cell. The gene is obtained by extracting a genomic DNA from a yeast, preparing a gene library, screening the resultant gene library and selecting the objective DNA.;COPYRIGHT: (C)1997,JPO