A method for the production of serum anti Hy for sexing Embryos, the antiserum produced and a method for its use.

摘要

The antiserum was obtained by Immunization and H - Wistar females with male Spleen cells of the same Strain (White et al. 1982).The Spleen Cell suspension is prepared by Mechanical Dissociation using a homogenizadord with tissue culture medium and PBS (phosphate buffer saline).The supernatant is once again centrifuged at 1000 g for 20 minutes at 4oC and the final pellet is resuspended in 5 ml of cultivopbs. With medium.The concentraciu00f3nfinal d e cells is determined by counting in a Neubauer Chamber.The Immunization Procedure of the females will be carried out by repeated intraperitoneal injections of 50 million Spleen cells.A total of nueveinyeccion is to losumo will be administered in three weeks. Subsequently two Booster injections were administered every two weeks.Seven days after the second Booster Injection, elantisuero females will be obtained by extraction of previamenteantestesiadas 1.00 ml of blood from the retro orbital Sinus.Then the seru00e1tratado antiserum to 56u00b0C for 30 minutes to remove the add-on.The Serum anti Hy produced by this method and the method of application of dichosuero to obtain Progeny selected.