Method for identifying agonists and antagonists of dna replication using an (in vitro) mammalian dna replication system

摘要

The present invention relates to an in vitro mammalian DNA replication system and to a method for identifying agonists and antagonists of DNA replication. The method comprises contacting in vitro a plasmid with a mixture comprising nuclear or cytoplasmic extracts from HeLa cells, the drug and a mixture of nucleotides, assessing the stimulation or the inhibition of initiation of DNA replication and the elongation of nascent DNA produced by the drug, and identifying the essential structures of the drug by quantitative structure-activity relationship (QSAR) analysis deriving relationships between the structural features of the drug and biological responses produced by the binding of the drug to the target receptor. The plasmid has a target receptor and comprises a specific mammalian origin of DNA replication.