Chromosome transfer (xmmct) to es cells, induced during the exposure of microcells to radiation

摘要

The present invention discloses a method for producing a transgenomic ES cell comprising the whole or a part of a heterologous chromosome. The method comprises the steps of: a) tagging a chromosome or a part of a chromosome in a cell with a dominant selectable marker; b) inducing microcell formation in the cell; c) isolating the microcells and irradiating them; d) fusing the microcells to ES cells; e) selecting for hybrids comprising the chromosome or part of the chromosome.