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Penicllin binding protein 2A from staphylococcus aureus strain 27R and derivatives for use in purification therefor and assay for compounds effective against methicillin resistant organisms
Penicllin binding protein 2A from staphylococcus aureus strain 27R and derivatives for use in purification therefor and assay for compounds effective against methicillin resistant organisms
The invention provides a gene encoding a novel PBP2A penicillin binding protein isolated from the methicillin resistant S. aureus strain 27R. The invention further provides soluble forms of PBP2A proteins lacking the transmembrane association region yet retain their active site configuration of the PBP2A protein. The invention also provides a method of assaying for agents useful against methicillin resistant staphylococcus strains by creating a kinetically inert complex between a support-immobilized transition metal ion and a modified soluble form of the PBP2A protein incorporating a chelating agent. The invention further provides a cell free method of assaying for agents which bind the PBP2A-27R protein.
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机译:本发明提供了编码从耐甲氧西林的 S I>分离的新型PBP2A青霉素结合蛋白的基因。 金黄色葡萄球菌 I>菌株27R。本发明进一步提供了缺乏跨膜结合区但仍保留其PBP2A蛋白活性位点构型的PBP2A蛋白可溶形式。本发明还提供了一种通过在支持物固定的过渡金属离子和结合螯合剂的PBP2A蛋白的修饰的可溶形式之间形成动力学惰性的复合物来测定对甲氧西林抗性葡萄球菌菌株有用的试剂的方法。本发明进一步提供了一种测定结合PBP2A-27R蛋白的试剂的无细胞方法。
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