DNA AND AMINO ACID SEQUENCE OF PENICILLIN BINDING PROTEIN 2ADERIVED FROM STAPHYLOCOCCUS AUREUS STRAIN 27 R, DERIVATIVE USED FOR ITS PURIFICATION AND ASSAY OF COMPOUND EFFECTIVE AGAINST MESITYLENE-RESISTANT LIVING ORGANISM

摘要

The invention provides a gene encoding a novel PBP2A penicillin binding protein isolated from the methicillin resistant S. aureus strain 27R. The invention further provides soluble forms of PBP2A proteins lacking the transmembrane association region yet retain their active site configuration of the PBP2A protein. The invention also provides a method of assaying for agents useful against methicillin resistant staphylococcus strains by creating a kinetically inert complex between a support-mobilized transition metal ion and a modified soluble form of the PBP2A protein incorporating a chelating agent. The invention further provides a cell free method of assaying for agents which bind the PBP2A-27R protein.