Prevention and treatment of mastitis in cows using chitosan or chitosan oligosaccharide as an active ingredient

摘要

(Objective) An object of the present invention is to provide a method for developing a novel cow mastitis treatment.;(Composition) In order to enhance the antimicrobial activity of chitosan derivatives, which are known to have immune cell activity and antibacterial effect, the physical properties with high molecular weight distribution of chitosan oligosaccharides were selected and the purity of chitosan on the antimicrobial activity of chitosan oligosaccharides was determined. More than 90% of chitosan oligosaccharides were isolated and used to investigate the therapeutic effects of mastitis, the most economically damaging in the dairy industry. In the present invention, first, in order to confirm the antimicrobial effect of runaway mastitis-inducing bacteria under laboratory conditions, the result was treated by concentration and time, and as a result, when the addition of 0.0001% or more of chitosan oligosaccharides showed a killing effect when more than 5 minutes passed, 18 Over time, the surviving colonies did not appear and most died. In addition, in view of the above results, after artificially infecting an animal with S. aureus causing mastitis, the chitosan oligosaccharide was administered in vivo, and the treatment effect was examined, and the survival rate was less than 10% in the animals not treated with chitosan oligosaccharide. In comparison, chitosan preparations had a therapeutic effect of 70-100%.;Based on the results, mastitis survival rate orally administered 15 g of chitosan oligosaccharides to infected cows for 7 days or more daily, activating monocytes and killing bacteria and reducing somatic cell count in milk, which play an important role in immune response. I was.