Methods for producing human interleukin-2 protein in potatoes and for promoting the growth of potato by interleukin-2 transformants.

摘要

The present invention relates to a method for inhibiting the activity of interleukin-2 produced from potatoes using animal cells that are dependent on interleukin-2 by expressing human interleukin-2 protein in a higher plant based on potatoes with rapid regeneration characteristics The present invention relates to a method for promoting the growth of used potatoes. The inventors have searched for a system capable of mass-producing useful proteins using potatoes which are less costly than the method for producing human interleukin-2 by tobacco plants, 2 gene of the present invention has an effect on a plant, particularly a potato, to increase industrial utility value, and to track the regulation mechanism of gene expression. The present invention relates to a 35S CaMV promoter (vector) using vector pSSK-) And neomycin phosphotransferase II (NPT II) which is a marker for selection of transformants. And the pBI121 vector carrying the GUS (-glucuronidase) gene was manipulated to replace the GUS (-glucuronidase) gene with the interleukin-2 gene. This gene was introduced into the present invention using Agrobacterium, To produce human interleukin-2 protein and to stimulate the growth of potato by interleukin-2 transformants in a potato as shown in Fig. 1, in which the interleukin-2 gene is expressed in potato as follows.