Human Interleukin-2 Protein Production from Potatoes

摘要

The present invention expresses the activity of human interleukin-2 protein in higher plants based on potatoes having fast regeneration characteristics, and thus shows the activity of interleukin-2 produced in potatoes using animal cells that depend on interleukin-2. The present invention relates to the growth promotion of used potatoes, and to search for a system for mass production of useful proteins using potatoes that are less expensive than the production method of human interleukin-2 by tobacco plants, and to produce gene carriers. We want to analyze the effects of genes on plants, especially potatoes, and to increase the industrial utility, while tracking the mechanisms of gene expression. The present invention uses the vector pSSK-1 to facilitate the 35S CaMV promoter. And neomycin phosphotransferase II (NPT II), a marker for selection of transformants. In addition, by manipulating the pBI121 vector containing the GUS (β-glucuronidase) gene and replacing it with the interleukin-2 gene instead of the GUS (β-glucuronidase) gene, the present invention uses Agrobacterium, which is a plant infection bacterium. A method for producing human interleukin-2 protein and potato growth by interleukin-2 transformants in potatoes that allow the expression of interleukin-2 gene in potatoes using the regeneration conditions established by