OPTIMIZED FERMENTATION PROCESSES FOR THE PREPARATION OF LIPOCORTINS IN E-COLI

摘要

the invention describes the manufacture of fremdproteinen optimized fermentationsverfahren in e. coli using the lac promoter or improved lac promoter (e.g. tac, trc).after the anwachsphase with kohlenstoffquelle glucose is the induction of the produktbildung (1) on iptg under glucose limitation or (2) on lactose in lactose limitation or (3) on iptg and lactose in lactose limitation. the limitation of glucose or lactose is designed so that the oxygen partial pressure above 10%.