Oligonucleotides for human HLA-A and HLA-B subtyping

摘要

At least one of the following oligonucleotides are used for the reverse-transcription of human HLA-A and HLA-B RNA and for amplification and sequencing of the resulting cDNA: (1) 5' CCC ATC TCA GGG TGA 3' (2) 5' GCT CCC ACT CCA TGA GGT ATT TC 3' (3) 5' ATC TCA GGG TGA GGG GCT TG 3' (4) 5' TGC CCA CTT CTG GAA GGT TCT 3' (5) 5' CTC GCT CTG GTT GTA G 3' (6) 5' TTC CCG TTC TCC AGG T 3' (7) 5' TGC TGG CCT GGT CTC CAC 3'.