Method for large-scale propagation of trees of genus Gmelina by tissue culture

摘要

Plantlets of trees of genus Gmelina can be efficiently regenerated on a large scale by culturing shoot apexes of trees of genus Gmelina on a B5 medium or its modified medium, each containing benzylaminopurine (BAP) or indole-3-butyric acid (IBA) together with BAP as plant growth regulators, thereby inducing and proliferating multiple shoots each having a large number of either definite buds or adventitious buds or both, then producing a large number of shoots from the resulting multiple shoots, transplanting the shoots to a B5 medium or its modified medium containing no plant growth regulator or containing both BAP and IBA as plant growth regulators; and the regenerated plantlets can be efficiently acclimatized to outdoor conditions by transplanting the regenerated plantlets to a mixture of river sands and crushed brick pieces in a mixing ratio of 1:1 by volume and acclimatizing the transplanted plantlets to outdoor conditions in an acclimatization box; and in this manner, trees of genus Gmelina can be propagated on a large scale.