METHOD FOR LARGE-SCALE PROPAGATION OF TREES OF GENUS GMELINA BY TISSUE CULTURE

摘要

PLANTLETS OF TREES OF GENUS GMELINA CAN BE EFFICIENTLY REGENERATED ON A LARGE SCALE BY CULTURING SHOOT APEXES OF TREES OF GENUS GMELINA ON A B5 MEDIUM OR ITS MODIFIED MEDIUM, EACH CONTAINING BENZYLAMINOPURINE (BAP) OR INDOLE-3-BUTYRIC ACID (LBA) TOGETHER WITH BAP AS PLANT GROWTH REGULATORS, THEREBY INDUCING AND PROLIFERATING MULTIPLE SHOOTS EACH HAVING A LARGE NUMBER OF EITHER DEFINITE BUDS OR ADVENTITIOUS BUDS OR BOTH, THEN PRODUCING A LARGE NUMBER OF SHOOTS FROM THE RESULTING MULTIPLE SHOOTS, TRANSPLANTING THE SHOOTS TO A B5 MEDIUM OR ITS MODIFIED MEDIUM CONTAINING NO PLANT GROWTH REGULATOR OR CONTAINING BOTH BAP AND LBA AS PLANT GROWTH REGULATORS; AND THE REGENERATED PLANTLETS CAN BE EFFICIENTLY ACCLIMATIZED TO OUTDOOR CONDITIONS BY TRANSPLANTING THE REGENERATED PLANTLETS TO A MIXTURE OF RIVER SANDS AND CRUSHED BRICK PIECES IN A MIXING RATIO OF 1 : 1 BY VOLUME AND ACCLIMATIZING THE TRANSPLANTED PLANTFETS TO OUTDOOR CONDITIONS IN AN ACCLIMATIZATION BOX; AND IN THIS MANNER, TREES OF GENUS GMELINA CAN BE PROPAGATED ON A LARGE SCALE. (FIGURE 5)