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Method for amplification and expression of nucleic acids in solid media and its application for nucleic acid cloning and diagnostics
Method for amplification and expression of nucleic acids in solid media and its application for nucleic acid cloning and diagnostics
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机译:在固体培养基中扩增和表达核酸的方法及其在核酸克隆和诊断中的应用
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摘要
Amplification and/or expression of nucleic acids is carried out in a medium immobilized by using an organic and/or inorganic solid matrix penetrating the medium and having a porous, fibrous, reticulated, coiled, capillary, lamellar or folded texture and which includes the components of a cell-free enzyme system of exponential amplification of nucleic acids and/or components of a cell-free enzyme system of nucleic acid expression. In this medium, the progeny of each molecule (clone) and the expression products remain in the same zone of the reaction volume where the matrix molecule was initially located. The method permits cloning of nucleic acids in vitro as well as detection of solitary nuleic acid molecules in the sample studied.
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