Cell-free system for DNA replication initiation

摘要

PCT No. PCT/GB97/01751 Sec. 371 Date Jan. 25, 1999 Sec. 102(e) Date Jan. 25, 1999 PCT Filed Jun. 26, 1997 PCT Pub. No. WO97/49797 PCT Pub. Date Dec. 31, 1997A cell-free system for initiating DNA replication under cell cycle control includes S phase cytosol or a fraction thereof in which are incubated G1 phase nuclei, which are co-incubated with S phase nuclei or a fraction thereof and/or cyclins A and/or E complexed to their cognate cyclin dependent kinase (Cdk2). The system may be used to assay for substances which modulate DNA synthesis or initiation thereof, and which have therapeutic potential in a number of contexts.