Cell-free system for initiation of DNA replication

摘要

A cell-free system for initiating DNA replication under cell cycle control includes S phase cytosol or a fraction thereof in which are incubated G1 phase nuclei, which are co-incubated with S phase nuclei or a fraction thereof and/or cyclins A and/or E complexed to their cognate cyclin dependent kinase (Cdk2). The system may be used to assay for substances which modulate DNA synthesis or initiation thereof, and which have therapeutic potential in a number of contexts.