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DETECTION OF MISMATCH IN DOUBLE-STRANDED DNA, DETECTION OF NUCLEIC ACID HAVING MUTATION, AND SEPARATION OF DOUBLE-STRANDED DNA HAVING MISMATCH
DETECTION OF MISMATCH IN DOUBLE-STRANDED DNA, DETECTION OF NUCLEIC ACID HAVING MUTATION, AND SEPARATION OF DOUBLE-STRANDED DNA HAVING MISMATCH
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机译:双链DNA错配的检测,核酸突变的检测和双链DNA错配的分离
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摘要
PROBLEM TO BE SOLVED: To provide a method for efficiently detecting any mismatch in nucleic acid using MutM protein by contacting subject double-stranded nucleic acid with MutM protein, followed by detecting any binding of the double-stranded nucleic acid to the protein. SOLUTION: This is a method for efficiently detecting any mismatch in double-stranded nucleic acid utilizing MutM protein having an ability of detecting all the mismatches including cytosine in nucleic acid by contacting subject double-stranded nucleic acid which is bound to a support or is labelled so as to be able to bind to a support with MutM protein which is derived from Escherichia coli, and is detectably labelled, binding the double-stranded nucleic acid to MutM protein, followed by detecting any binding of the subject double- stranded nucleic acid to MutM protein. It is also possible to efficiently separate double-stranded nucleic acid containing mismatch(es) from double-stranded nucleic acid containing no mismatch.
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