Detection manner of the gene due to fluorescent ellipsometric method and method of detection of the Vero toxin production germ

摘要

Abstract Topic At the time of detecting the gene of multiple genera in sample, the glowflyRepeatability to be good making use of optical ellipsometric method, the accuracy and brief quicklyMaking use of manner, and the said manner which it detects the V of O157 and the likeThe manner which detects the presence of the ero toxin production germ quickly is offeredru. Solutions The gene of the specification which is included in the sample the geneTo amplify with amplification modulo, the said specific gene dosage in the said amplification productAt fluorescent ellipsometric method by measuring the said specific geneThe multiple genera which are included in the sample in the manner which detects presence,The gene making use of the universal primer simultaneously amplificationAfter doing, each one unique vis-a-vis the gene of the said multiple genera cupMaking use of the fluorescent indicator reagent of the multiple genera which it adjusts the gene of the said multiple generaThe respective tonnage is measured simultaneously.