The invention relates to a DNA oligonucleotide which is specific for Meloidogyne species, which DNA oligonucleotide has the following sequence: (a) a DNA sequence chosen from the group consisting of SEQ ID No. 1 to SEQ ID No. 20, or (b) a DNA sequence which under conditions of stringency hybridises with a sequence according to (a) and which is specific for Meloidogyne species. Furthermore, the invention relates to a DNA vector containing such a DNA oligonucleotide, as well as to a host cell containing such vector. The invention also relates to a process for determining the presence of a Meloidogyne species, according to which DNA is isolated from a sample to be analysed, and is subsequently subjected to a PCR reaction, using a DNA oligonucleotide according to the invention. Finally, the invention relates to a kit for determining the presence of a Meloidogyne species.
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机译:本发明涉及一种对根结线虫物种具有特异性的DNA寡核苷酸,该DNA寡核苷酸具有以下序列:(a)选自SEQ ID No.1至SEQ ID No.20的DNA序列,或(b)该DNA序列在严格条件下与根据(a)的序列杂交,并且对根结线虫物种具有特异性。此外,本发明涉及含有这种DNA寡核苷酸的DNA载体,以及含有这种载体的宿主细胞。本发明还涉及确定根结线虫种类的存在的方法,根据该方法,从待分析的样品中分离DNA,然后使用根据本发明的DNA寡核苷酸进行PCR反应。最后,本发明涉及一种用于确定根结线虫物种的存在的试剂盒。
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